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Abstract Grass leaves develop from a ring of primordial initial cells within the periphery of the shoot apical meristem, a pool of organogenic stem cells that generates all of the organs of the plant shoot. At maturity, the grass leaf is a flattened, strap-like organ comprising a proximal supportive sheath surrounding the stem and a distal photosynthetic blade. The sheath and blade are partitioned by a hinge-like auricle and the ligule, a fringe of epidermally derived tissue that grows from the adaxial (top) leaf surface. Together, the ligule and auricle comprise morphological novelties that are specific to grass leaves. Understanding how the planar outgrowth of grass leaves and their adjoining ligules is genetically controlled can yield insight into their evolutionary origins. Here we use single-cell RNA-sequencing analyses to identify a ‘rim’ cell type present at the margins of maize leaf primordia. Cells in the leaf rim have a distinctive identity and share transcriptional signatures with proliferating ligule cells, suggesting that a shared developmental genetic programme patterns both leaves and ligules. Moreover, we show that rim function is regulated by genetically redundant Wuschel-like homeobox3 (WOX3) transcription factors. Higher-order mutations in maizeWox3genes greatly reduce leaf width and disrupt ligule outgrowth and patterning. Together, these findings illustrate the generalizable use of a rim domain during planar growth of maize leaves and ligules, and suggest a parsimonious model for the homology of the grass ligule as a distal extension of the leaf sheath margin. 

In this study, a methodology for designing a task-based exoskeleton which can recreate the end-effector trajectory of a given limb during a rehabilitation task/movement is presented. The exoskeleton provides an option to replace traditional joint-based exoskeleton joints, which often have alignment issues with the biological joint. The proper fit of the exoskeleton to the user and task are research topics to reduce pain or joint injuries as well as for the execution of the task. The proposed task-based synthesis method was successfully applied to generate the 3D motions of the elbow flexion and extensions using a one degree of freedom (DOF), spatial four-bar mechanism. The elbow joint is analyzed through motion capture system to develop the bio-exoskeleton. The resulted exoskeleton does not need to align with the corresponding limb joint to generate the desired anatomical motion.